The Perfect Stage and SSR Analysis of Magnaporthe grisea in the Yanxi Blast Nursery, Hunan Province

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The Perfect Stage and SSR Analysis of Magnaporthe grisea in the Yanxi Blast Nursery, Hunan Province

SHEN Ying^{1; 2}; Julien FROUIN ²; HE Yue-qiu³; Claudia KAYE ²; XIAO Fang-hua⁴; Jean Loup NOTTEGHEM⁵; LIU Er-ming⁴; Didier THARREAU²

( ¹ State Key Laboratory of Rice Biology; China National Rice Research Institute; Hangzhou 310006; China; ²Centre de Cooperation Internationale en Recherche Agronomique pour le Developement; TA 73 / 09; 34398 Montpellier; Cedex 05; France; ³Key Laboratory for Plant Pathology of Yunnan Province; Yunnan Agricultural University; Kunming 650201; ⁴Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha)

Received:1900-01-01 Revised:1900-01-01 Online:2004-05-10 Published:2004-05-10

湖南烟溪病圃稻瘟病菌的有性态及微卫星标记的遗传多样性分析

沈瑛1，2; Julien FROUIN 2; 何月秋3 ; Claudia KAYE 2;肖放华4；Jean Loup NOTTEGHEM5；刘二明4；Didier THARREAU 2

1中国水稻研究所，水稻生物学国家重点实验室，浙江杭州 310006

Abstract

Abstract: One hundred and twenty-four Magnaporthe grisea isolates collected in four years from the Yanxi Blast Nursery, Hunan Province were tested for mating type with four hermaphroditic fertile isolates MAT1.1 (KA3 and TH12) and MAT1 2 (Guy11 and TH16) and the genetic diversity of 105 isolates were analyzed by using microsatellite markers(SSR). Among the 124 isolates, 28 belonged to MAT1.1, 39 to MAT1.2 and the remaining 57 to sterile ones, taking 22.58%, 31.45% and 45 97% for each group, respectively. The 105 isolates could be divided into six lineages at 0.67 similarity level based on the amplicons with seven pairs of SSR primers.

Key words: Magnaporthe grisea, hermaphroditic isolate, microsatellite marker, mating type, genetic diversity

摘要： 用2对稻瘟病菌的两性能育菌株（交配型1.1和交配型1.2）测定了来自湖南烟溪病圃上的4年124个稻瘟病菌株的交配型，用微卫星分子标记分析其中105个菌株的遗传多样性及其亲缘关系。 124个菌株中， 28个为交配型1.1, 39个为交配型1.2， 57个不育, 分别占供试菌株的22.58%、31.45%和45.97%；在0.67相似性的基础上，7对微卫星引物扩增出的标记可将105个菌株区分为6个系谱群。

关键词: 稻瘟病菌, 两性菌株, 微卫星标记, 交配型, 遗传多样性

SHEN Ying ,Julien FROUIN ,HE Yue-qiu ,Claudia KAYE ,XIAO Fang-hua ,Jean Loup NOTTEGHEM ,LIU Er-ming ,Didier THARREAU . The Perfect Stage and SSR Analysis of Magnaporthe grisea in the Yanxi Blast Nursery, Hunan Province [J]. Chinese Journal of Rice Science.

沈瑛, Julien FROUIN , 何月秋 , Claudia KAYE ,肖放华,Jean Loup NOTTEGHEM,刘二明,Didier THARREAU . 湖南烟溪病圃稻瘟病菌的有性态及微卫星标记的遗传多样性分析[J]. 中国水稻科学.

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