Chinese Journal of Rice Science

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Somaclonal Male-Sterile Mutants and Their Expressions in Indica Rice (in English)

Ling Dinghou; Liang Chengye; Ma Zhenrong; Chen Meifang; He Bingsen   

  • Received:1900-01-01 Revised:1900-01-01 Online:1990-03-17 Published:1990-03-17

籼稻体细胞无性系雄性不育变异及其表现(英文)

凌定厚;粱承邺;马镇荣;陈梅芳;何炳森   

  1. 中国科学院华南植物研究所,广州510650

Abstract: In past five years from 1984 to 1988, 48 cases of male sterile (ms) mutants in nine varieties were found, in which 30 cases belonged to R1 generation and 28 to R2 generation. The frequency of ms variation in R, generation was around 1% and 2% in R2 generatiom. According to the characters of ms-mutants and their genetic expressions,pollen grain free(nuclear sterile) and abortive pollen (cytoplasmic genetic sterile) could be classified, In 1R54, two cases of pollen grain free ms-mutants were discovered similar in different generations (R1 and R2 generation) and different years (1985 and 1986) . Among ms-mutants of the abortive pollen type, one (54257) was found to be the cytoplasmic genetic male sterile mutant. Zheng-Shan 97, Er-Jiu-Ai,162-5 (a stable somaclonefrom IR52) and Gui-Chao 2 could maintain male sterility when crossed to the ms line (54257). IR24, IR36, IR54,Shuang-Er-Zhan and others could restore its fertility. This result showed that "54257" was a cytoplasmic ms-mutant. This is the first sample that the cytoplasmic ms mutant could be induced by tissue culture in the world.

Key words: Indica rice, Plant tissue culture, Somaclonal variation

摘要: 通过组织培养,于1984一1988年间,在9个品种(14次)中离体筛选到雄性不育突变件48个,其中发生在R1代的20个,R2代的28个。根据花粉败育之特点,可将这些突变体分为无花粉型及花粉败育型。发现起源于IR54的一体细胞无性系“54257”,在其R2代分离出雄性不育突变体,继之对它以各种品种进行测交,其杂种一代有的组合表现育性恢复,有的组合仍保持雄性不育。由此确证它属核质互作型雄性不育。该雄性不育突变体,就其恢保关系考察,初步认为其细胞质性质与野败型(WA型)类似。这是由体细胞无性变异产生核质互作型雄性不育的首例报道。

关键词: 籼稻, 植物组织培养, 体细胞无性系变异