Mapping and Genetic Analysis of Quantitative Trait Loci for Related Traits of Cooked Rice

doi:10.3969/j.issn.10017216.2011.05.004

Abstract

Abstract: The milled rice length, cooked rice length and elongation traits are very important in the quality of cooked rice. Mapping of quantitative trait locus (QTL) for cooked rice related traits was conducted using a doubled haploid population from a cross between a typical indica rice cultivar ‘TN1’ and a typical japonica rice cultivar ‘Chunjiang 06’. Using a linkage map based on 177 SSR molecular marker loci covering the distance of 1670.92 cM, a total of 14 QTLs were identified for the cooked rice related traits. One putative QTL for milled rice length and one for soaked rice length were mapped on chromosome 2, with the explained variance of 15.20% and 18.50%, respectively. One putative QTL for soaked rice expansion was mapped on chromosome 6, with the explained variance of 13.39%. One putative QTL for boiled rice length was mapped on chromosome 9, with the explained variance of 1360%. Three QTLs for steamed rice length were detected on chromosomes 1, 3 and 12, totally explaining variance of 45.00%. Four QTLs for boiled rice elongation were detected on chromosomes 3, 6, 9 and 10, totally explaining variance of 61.30%. Three QTLs for steamed rice elongation were detected on chromosomes 1, 3, and 6, totally explaining variance of 49.10%. Two QTLs for elongation related traits of cooked rice were detected on the Wx gene cluster and ALK gene cluster, respectively. Comparing to the Wx gene cluster, the QTL in the ALK gene cluster played a much more important role in regulation of related traits of cooked rice with the LOD score of 6.35. These results provide a foundation for further cloning of genes that regulate cooked rice related traits and a theoretical basis for the improvement of rice cooking quality and markerassisted breeding of highyielding and highquality rice varieties.

Key words: ricefont-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">;related traits of cooked ricefont-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">;quantitative trait locusfont-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">;doubled haploid population

摘要： 米粒长、饭粒长和饭粒延伸系数等性状与米饭品质密切相关。以籼稻台中本地1号（TN1）与粳稻春江06为亲本构建的加倍单倍体群体为材料，利用全基因饱和分子标记连锁遗传图谱对多个稻米出饭特性相关的性状进行QTL定位, 共检测到14个QTL。其中，米粒长和米粒浸长QTL各1个，均位于第2染色体上，分别可以解释性状变异的15.20% 和1850%；1个米粒浸泡膨胀率QTL，位于第6染色体上，可解释性状变异的1339%；1个煮饭粒长QTL，位于第9染色体上，可解释性状变异的1360%； 3个蒸饭粒长QTL，分别位于第1、3和12染色体上，共解释性状变异4500%；4个煮饭延伸率QTL，分别位于第3、6、9和10染色体上，共解释性状变异6130%； 3个蒸饭延伸率QTL分别位于第1、3和6染色体上，共解释性状变异4910%。在已知的Wx和ALK基因所在区域都检测到了米饭延伸性相关的QTL。相比较而言，覆盖ALK基因的QTL对出饭特性的影响更大，LOD值达到了635。该研究结果可为稻米出饭特性相关调控基因的克隆奠定基础，同时对稻米品质的改良及高产优质稻品种的分子标记辅助选育提供理论参考。

关键词: 水稻font-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">; 出饭特性font-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">; 数量性状基因座font-family: 宋体, mso-bidi-font-family: 宋体, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">;加倍单倍体群体

CLC Number:

SHEN Nianwei1,#, LAI Kaikai1,2,#, NIAN Jinqiang1,3, ZENG Dali1, HU Jiang1, GAO Zhenyu1, GUO Longbiao1, ZHU Li1, LIU Jian1, DONG Guojun1, YAN Meixian1, QIAN Qian1,*, ZHANG Guangheng1,* . Mapping and Genetic Analysis of Quantitative Trait Loci for Related Traits of Cooked Rice[J]. Chinese Journal of Rice Science, 2011, 25(5): 475-482.

沈年伟1,#,来凯凯1,2,# ,粘金沯1,3 ,曾大力1,胡江1,高振宇1,郭龙彪1,朱丽1,刘坚1, 董国军1,颜美仙1,钱前1,*,张光恒1,* . 稻米出饭特性QTL分析及遗传研究[J]. 中国水稻科学, 2011, 25(5): 475-482.

References

\[1\]汤圣祥. 我国杂交水稻蒸煮与食用品质的研究. 中国农业科学, 1987, 20(5): 1722.

\[2\]田志喜, 严长杰, 钱前, 等. 水稻淀粉合成相关基因分子标记的建立. 科学通报, 2010, 55(26): 25912601.

\[3\]何予卿, 邢永忠, 葛小佳. 水稻米饭延伸指数相关性状的基因定位研究. 分子植物育种， 2003, 1(5): 613619.

\[4\]Wang Z Y, Zheng F Q, Shen G Z, et al. The amylose content in rice endosperm is related to the posttranscriptional regulation of the waxy gene. Plant J, 1995, 7: 613622.

\[5\]Sano Y. Differential regulation of waxy gene expression in rice endosperm.Theor Appl Genet, 1984, 68: 45674573.

\[6\]Mikami I, Uwatoko N, Ikeda Y, et al.Allelic diversification at the wx locus in landraces of Asian rice. Theor Appl Genet, 2008, 116(7): 979989.

\[7\]高振宇, 曾大力, 崔霞, 等. 水稻稻米糊化温度控制基因ALK的图位克隆及其序列分析. 中国科学C辑: 生命科学, 2003, 33(6): 481487.

\[8\]沈圣泉, 庄杰云, 王淑珍, 等. 水稻米粒延伸性QTLs 定位和基因型与环境互作分析. 中国水稻科学, 2005, 19(4): 319322.

\[9\]包劲松, 谢建坤, 夏英武. 籼稻米粒延伸性的遗传研究. 作物学报, 2001, 27(4): 489492.

\[10\]Hussain A A, Maurya D M,Vaish C P. Studies on quality status of indigenous upland rice (Oryza sativa). Indian J Genet， 1987, 47: 145.

\[11\]Vivekanandan P, Girdharan S. Genetic variability and character association for kernel and cooking quality traits in rice.Oryza, 1998, 35(3): 242245.

\[12\]Ahn S N, Bollich C N, McClung A M, et al. RFLP analysis of genomic regions associated with cookedkernel elongation in rice. Theor Appl Genet, 1993, 87: 2732.

\[13\]张光恒, 曾大力, 郭龙彪, 等. 水稻米粒延伸性的遗传剖析. 遗传, 2004, 26(6): 887892.

\[14\]Tan Y F, Xing Y Z, Li J X, et al.Genetic bases of apperance quality of rice grains in Shanyou 63, an elite rice hybrid. Theor Appl Genet, 2002, 101: 823829.

\[15\]Liu L L, Yan X Y, Jiang L, et al.Identification of stably expressed quantitative trait loci for cooked rice elongation in nonBasmati varieties. Genome, 2008, 51(2): 104112.

\[16\]姜树坤, 黄成, 徐正进, 等. 粳稻米粒延伸性的QTL剖析. 植物生理学通讯, 2008, 44(6): 10911094.

\[17\]朱永义, 周显青, 赵秀萍. 米粒吸水特性及其影响因素的研究. 粮食与饲料工业, 1998（3）: 1314.

\[18\]莫紫梅, 许金东, 赵思明. 米饭品质的研究进展. 粮食与饲料工业, 2008（11）: 58.

\[19\]杨长登, 郭龙彪, 李西明, 等. 水稻抗恶苗病微效QTL的定位. 中国水稻科学, 2006, 20(6): 657659.

\[20\]农业部部颁标准NY14788. 米质量测定方法. 北京: 中国标准出版社, 1988.

\[21\]Wang D L, Zhu J, Li Z K, et al.Mapping QTLs with epistatic effects and QTL ×environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99(7/8): 12551264.

\[22\]张玉山, 汪剑波, 徐才国, 等. 水稻5个形态性状显著相关性的分子基础剖析. 科学通报, 2010, 55(17): 16821689.

\[23\]邢永忠，谈移芳，徐才国, 等. 利用水稻重组自交系群体定位谷粒外观性状的数量性状基因. 植物学报, 2001, 43(8): 840845.

\[24\]Ge X J, Xing Y Z, Xu C G, et al.QTL analysis of cooked rice grain elongation, volume expansion, and water absorption using a recombinant inbred population. Plant Breeding, 2005, 124: 121126.

\[25\]彭勃, 王阳, 李永祥, 等. 不同水分环境下玉米产量构成因子及籽粒相关性状的QTL分析. 作物学报, 2010, 36(11): 18321842.

\[26\]毛艇, 徐海, 郭艳华, 等. 籼粳交重组自交系的亚种属性与稻米品质性状的关系. 中国水稻科学, 2010, 24(5): 474478.

\[27\]Messmer R, Fracheboud Y, Banziger M, et al.Drought stress and tropical maize: QTLby environment interactions and stability of QTLs across environments for yield components and secondary traits. Theor Appl Genet, 2009, 119: 913930.

\[28\]Hallauer A R, Miranda J B. Quantitative Genetics in Maize Breeding. Iowa: Iowa State University Press, 1981.

\[29\]Li H, Ribaut J M, Li Z, et al.Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations. Theor Appl Genet, 2008, 116: 243260.

[1]	JIN Yi1,2 , LIU Heqin1 , WANG Dekai1，,TAO Yuezhi1,. Genetic Analysis and Gene Mapping of a White Striped Leaf and White Panicle Mutant in Rice [J]. Chinese Journal of Rice Science, 2011, 25(5): 461-466.
[2]	YU Hao, YAN Xu, GUO Weidong, XIN Dedong*. Single Nucleotide Polymorphism of MicroRNA in Rice [J]. Chinese Journal of Rice Science, 2011, 25(5): 467-474.
[3]	XU Jianjun1,2, ZHAO Qiang3, ZHAO Yuanfeng1, ZHU Lei1, XU Chenwu1, GU Minghong1, HAN Bin3, LIANG Guohua1,*. Mapping of QTLs for Flag Leaf Shape Using WholeGenome Resequenced Chromosome Segment Substitution Lines in Rice [J]. Chinese Journal of Rice Science, 2011, 25(5): 483-487.
[4]	ZHAO Haixin1,2 , YANG Limin2, CHEN Shuqiang1, JIANG Shukun3, HUANG Xiaoqun1, SHAN Lili1, PAN Guojun1,* . Effects of RowSpacing on Canopy Structure and Yield in Different Type Rice [J]. Chinese Journal of Rice Science, 2011, 25(5): 488-494.
[5]	FU Guanfu#, SONG Jian#, LIAO Xiyuan, ZHANG Xiufu, XIONG Jie, WANG Xi, LE Mingkai, TAO Longxing*. Thermal Resistance of Common Rice Maintainer and Restorer Lines to High Temperature Stress During Flowering and Early Grain Filling Stages [J]. Chinese Journal of Rice Science, 2011, 25(5): 495-500.
[6]	LU Yandong1,2, ZHENG Guiping2, GUO Xiaohong2, YIN Dawei1, MA Dianrong1, XU Zhengjin1, CHEN Wenfu1,*. Effects of Lower Limit of Soil Water Potential on Grain Quality of Rice in Cold Region [J]. Chinese Journal of Rice Science, 2011, 25(5): 515-522.
[7]	LONG Jirui1, MA Guohui1,*, WAN Yizhen1, SONG Chunfang1, SUN Jian2. Effects of Nitrogen Fertilizer Level on Chlorophyll Fluorescence Characteristic in Flag Leaf of Super Hybrid Rice at Late Growth Stage [J]. Chinese Journal of Rice Science, 2011, 25(5): 501-507.
[8]	HE Yangdong1,2, TONG Ping1， MA Jun1,*, SUN Yuanyuan3, SUN Yongjian1, LIU Shujin1, XU Yuanming4. Effects of Different SeedlingAges and Planting Densities on Physiological Characteristics and Grain Yield of Hybrid Rice ⅡYou 498 under TrianglePlanted System of Rice Intensification During Filling Stage [J]. Chinese Journal of Rice Science, 2011, 25(5): 508-514.
[9]	ZHANG Rongsheng1,2, CHEN Zhiyi1，*, LIU Yongfeng1. Genetic Diversity and Pathotype Variability of Xanthomans oryzae pv. oryzicola Strains [J]. Chinese Journal of Rice Science, 2011, 25(5): 523-528.
[10]	WANG Lihua, ZHANG Yueliang, GUO Huifang, FANG Jichao* . Comparison of Sublethal Effects of Chlorpyrifos on Resistant and Susceptible Populations of Laodelphax striatellus [J]. Chinese Journal of Rice Science, 2011, 25(5): 529-534.
[11]	HU Ning1, YAO Kemin1, ZHANG Xiaocui2, LU Chuangen3,*. Effect and Simulation of Plant Type on Canopy Structure and Radiation Transmission in Rice [J]. Chinese Journal of Rice Science, 2011, 25(5): 535-543.
[12]	SI Huamin, LIU Wenzhen, FU Yaping, SUN Zongxiu, HU Guocheng*. Current Situation and Suggestions for Development of TwoLine Hybrid Rice in China [J]. Chinese Journal of Rice Science, 2011, 25(5): 544-552.
[13]	AI Zhiyong1,2, MA Guohui1, QING Xianguo2，3,* . Physiological and Ecological Characteristics and Cultivation Regulation for High and Stable Yield of Super Hybrid Rice [J]. Chinese Journal of Rice Science, 2011, 25(5): 553-560.
[14]	WANG Bin1, LIU Hemei2, MAO Bigang3, GAO Suwei3, XU Hongbin1, GE Jiangui1,* . QTL Analysis on Apical Spikelet Abortion in Rice [J]. Chinese Journal of Rice Science, 2011, 25(5): 561-564.
[15]	E Zhiguo1, WANG Lei1,2,*. Construction of Database for Chinese Rice Varieties and Their Genealogy [J]. Chinese Journal of Rice Science, 2011, 25(5): 565-566.