Chinese Journal of Rice Science

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Study on Anther Culture Ability of different Ecotypes of O. rufipogon Griff

Pang Hanhua; Tang Shengxiang

  

  • Received:1900-01-01 Revised:1900-01-01 Online:1995-07-10 Published:1995-07-10

不同生态型普通野生稻花药培养力的研究

庞汉华1; 汤圣祥2   

  1. 1 中国农业科学院作物品种资源研究所,北京100081;2 中国水稻研究所品种资源系,杭州310006

Abstract: Thirty-seven entries of O. rufipogon from China including three ecotypes named as stolon, slope and erectness were studied on anther culture ability. The result indicated that there existed a obvious differences in anther culture ability in the tested entries, in which the callus induction rate ranged in 0-9.1%, and green plantlet differentiation in 0-32.1%. Pollen green seedlings were obtained in 31 of the 37 entries. The anther culture ability in order was as follows: cultivated rice>interspecific hybrids>erectness ecotype>slope ecotype>stolon ecotype. Different media showed different efficiency of anther culture to the tested ecotypes. However, improved N6-1 medium was the best one suitable to all three ecotypes of O. rufiporgon, which could lead to apparent increase in the efficiency of anther culture.

Key words: anther culture, ecotype, medium, Oryza rufipogon

摘要: 对我国匍匐、倾斜和直立型普通野生稻进行了花药培养力的研究。供试的37份普通野生稻其花药培养力存在明显差异,愈伤诱导率变幅0~9.1%,绿苗率变幅0~32.1%,其中有31份材料可得到花培绿苗,占83.8%%。花药培养力的高低顺序为:栽培籼稻>栽培稻/野生稻杂种>直立型普通野生稻>倾斜型普通野生稻>匍匐型普遍野生稻。不同培养基对不同生态型普遍野生稻具有不同的花培效率。改良N6一号培养基具有显著提高花培效率的作用,适合各型普通野生稻的花药培养。

关键词: 花药培养, 生态型, 培养基, 普通野生稻