Chinese Journal of Rice Science
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Guo Quansheng; Shen Ying
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过全生;沈 瑛
Abstract: Lower concentrations of 2,4-D (0. 2, 0. 4, 0. 8 mg/L) were used as auxins for induced calli of rice (Oryza sativa L.) on induction media. The results showed that 0.8 mg/L concentration of 2,4-D could induce a large number of calli earlier from scutellum or other parts, but calli could not differentiate green buds. On the contrary, 0.2 mg/L concentration of 2,4-D could only produce a few white, smoother and more compact granular structures or calli under the base of coleoptile, and the formation of them was very late, but the calli could change into clustered-buds and produce fine roots. These clustered-buds were cut into small pieces and transferred into the regeneration media containing KT, a large number of normal green plantlets (reached over 80%) were produced from the clustered-buds and the plantlets were transplanted on the field, they grew well and produced normal seeds.
Key words: dichlorophenoxyacetic acid, one-step induction method of test-tube seedlings, organ culture
摘要: 以较低浓度(0.2、0.4、0.8 mg/L)的2,4-D作为水稻(Oryza sativa L.)的外植体(种子)诱导愈伤组织培养基中的生长素类激素,发现0.8 mg/L的2,4-D能较早地使胚的盾片产生大量的愈伤组织,但这些愈伤组织在原培养基上不能分化出绿芽。含0.2 mg/L的2,4-D能在胚芽鞘节等部位产生一些较光滑、致密、白色颗粒状的结构或愈伤组织,虽然它们出现较迟,但这些结构物能在原诱导培养基上逐渐转化成芽簇,并产生一些纤细的根。将芽簇分割成小块,转移到加有KT等的成苗培养基上,能形成大量的幼苗,绿苗频率达80%以上,明显高于一般报道的水平。试管苗经大田种植后生长整齐一致,能产生出正常的种子。
关键词: 组织培养, 2, 4-D, 一次成苗
Guo Quansheng,Shen Ying. Study of Lower Concentration 2,4-D on Enhancement of Rice Green Plantlets in vitro[J]. Chinese Journal of Rice Science.
过全生,沈 瑛. 低浓度2,4-D提高水稻体细胞成苗研究[J]. 中国水稻科学.
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