Abstract: The mycelia and conidia of Phyllosticta oryzicola Hara could be used as inocula. The rice leaves could be infected by means of wound inoculations,and in which the clipping method is the simplest and most practical. But the unwounded inoculations were not successful. Also, the experiment showed that the rice leaves at the stages of seedling and tillering were resistant while those at the stages of booting, flowering and milk were susceptible. Ac cording to the results of the clipping inoculation at the booting-flowering stage of rice in the field, of 85 cultivars or hybrid combinations, 26 combinations of indica hybrid rice were almost susceptible or highly susceptible. There were significant differences in the resistance among 50 cultivars of medium indica rice,in which most cultivars mainly cultivated in Jiangsu were moderately susceptible or moderately resistant. 8 cultivars of japonica or glutinous rice were moderately resistant or resistant.

Key words: evaluation for resistance, inoculation techniques, Phyllosticta oryzicola

摘要： 以盆栽杂交稻汕优63为供试品种开展人工接种技术研究，并利用人工接种技术对85个品种（组合）进行了抗性鉴定。研究表明，水稻叶尖枯病菌的侵入途径主要是伤口。人工接种，以病菌分生孢子液（低倍镜视野15～30个孢子）或菌丝液，在水稻孕穗至扬花期进行剪叶蘸菌液接种为好。接种后一定时间内的病斑扩展长度可作为大田鉴定水稻品种抗性的评价标准。根据大田接种结果，不同稻型和品种对叶尖枯病的抗性差异较大。一般杂交籼稻感病，常规中籼稻次之，而粳、糯稻抗病。这一结果与水稻叶尖枯病的大田发生情况相似。抗性鉴定中发现，一般秆高、叶长且披垂的水稻品种（组合）较感病；抗病品种特别是粳糯稻品种在病健交界处表现明显的褐色过敏性反应。

关键词: 水稻叶尖枯病, 接种技术, 抗性鉴定