Abstract: A doubled haploid population, derived from anther culture of F₁ hybrid between a typical indica cv. Zhaiyeqing 8 and a japonica cv. Jingxi 17, has been used in this study. The chalkiness sizes of transverse section, flank section and belly section, were investigated in 127 DH lines with video microscopy and computer image analysis technique. The quantitative trait loci (QTLs) for the size of chalkiness were analysed. A total of nine QTLs were detected in five intervals of three chromosomes, including three QTLs for transverse section, three QTLs for flank section and three QTLs for belly section. The directions of additive effect of QTLs for three sections were identical, and the intervals and peak of LOD on three chromosomes were overlapping, some of them were uniform. The application value of video microscopy and computer image analysis technique in rice chalkiness analysis were discussed.

Key words: chalkiness, three-dimension section, doubled haploid lines, quantitative trait loci, video microscope

摘要： 以典型的籼／粳交（窄叶青8号/京系17）DH群体为材料，采用视频显微扫描技术和计算机图形分析相结合的技术手段，从横切面、侧面和腹面3个层面对127个DH株系的垩白大小进行了立体分析；并结合该群体业已构建的染色体图谱检测控制垩白的数量性状位点（QTL）。对横切面的分析检测到3个QTLs，分别位于第8、11和12染色体上的RZ617~G2132、ATT42B~RG98和CT462~RG574标记区间内。对侧面的分析也检测到3个QTLs，其LOD值的最高峰位点与横切面分析完全一致。对腹面的分析检测到3个QTLs, 分别位于第8、11和12染色体上的GA376~CT195、RZ638~RG304和CT462~RG574标记区间内，其加性效应来源与横切面和腹面分析的一致。3个切面的有关QTL的LOD值的分布峰区和最高峰位点的一致或相互覆盖也表明，应用视频显微扫描技术分析稻米垩白任一切面的数据均可定位控制垩白大小的QTL。

关键词: 稻米垩白, 三维切面, 加倍单倍体, 数量性状位点, 视频显微扫描技术